Use of Infrared Spectroscopy in Animal Studies: Aspects of Health Science

Non-invasive imaging technologies, such as infrared thermography (IRT), near-infrared spectroscopy (NIRS), and fourier-transform infrared spectroscopy (FTIR) are revolutionizing the field of animal research by enabling real-time, stress-free assessment of physiological conditions. Such methods like these are align with the ethical standards of the 3Rs, replacement, reduction, and refinement-by reducing the need of invasive procedures while enhancing data quality and animal welfare. These methods can also provide critical insight into animal health, behavior, and stress responses without the need for invasive procedures or restraint, thereby minimizing harm and distress. This literature review will explore the practical applications of IRT, NIRS, and FTIR in animal studies, including their role in pain detection, welfare monitoring, neurological research, and metabolic analysis. By bridging technological advancement with humane research practices, these tools support noy only the goal of research settings, but continue the evolution and implementation of infrared-based technologies that hold great promise for advancing health science in ways that benefit both animals and humans alike.